By Tara L. Fulton (auth.), Beth Shapiro, Michael Hofreiter (eds.)
Research into historic DNA all started greater than 25 years in the past with the ebook of brief mitochondrial DNA series fragments from the quagga, an extinct relative of the zebra. historic DNA study particularly won momentum following the discovery of PCR, which allowed hundreds of thousands of copies to be made up of the few ultimate DNA molecules preserved in fossils and museum specimens. In Ancient DNA: equipment and Protocols professional researchers within the box describe a number of the protocols which are now commonplace to check old DNA. those comprise directions for developing an historic DNA laboratory, extraction protocols for quite a lot of various substrates, info of laboratory ideas together with PCR and NGS library training, and recommendations for applicable analytical techniques to make experience of the sequences acquired. Written within the hugely profitable Methods in Molecular Biology™ sequence layout, chapters comprise introductions to their respective themes, lists of the required fabrics and reagents, step by step, effectively reproducible laboratory protocols, and key tips about troubleshooting and fending off identified pitfalls.
Authoritative and sensible, Ancient DNA: equipment and Protocols seeks to help scientists within the additional learn of old DNA and the methodological methods in old research.
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